467. Assay Conditions Significantly Alter AAV Neutralizing Antibody Determination

Application of a rapid microplaque assay for determination of human immunodeficiency virus neutralizing antibody titers.

To perform a human immunodeficiency virus (HIV) plaque assay in nonadherent host cells, we developed a novel technique in which HIV-infected MT-2 cells were formed into monolayers by centrifugation through molten agarose. Infection, formation of cell monolayers, and enumeration of plaques all took place in 96-well microtiter plates. When this process was preceded by 18 h of incubation of HIV wi...

AAV-Delivered Antibody Mediates Significant Protective Effects against SIVmac239 Challenge in the Absence of Neutralizing Activity

Long-term delivery of potent broadly-neutralizing antibodies is a promising approach for the prevention of HIV-1 infection. We used AAV vector intramuscularly to deliver anti-SIV monoclonal antibodies (mAbs) in IgG1 form to rhesus monkeys. Persisting levels of delivered mAb as high as 270 μg/ml were achieved. However, host antibody responses to the delivered antibody were observed in 9 of the 1...

Titration of Rinderpest Virus and Its Neutralizing Antibody

Pre-exposure Neutralizing Antibody

Maximilian Larena, Natalie A Prow, Roy A Hall, Nikolai Petrovsky and 6 Mario Lobigs 7 8 1 Department of Emerging Pathogens and Vaccines, John Curtin School of Medical 9 Research, The Australian National University, Canberra, Australia 10 2 Australian Infectious Diseases Research Centre, School of Chemistry and Molecular 11 Biosciences, The University of Queensland, St Lucia, Australia 12 3 Vaxi...

Use of anti-(+)-methamphetamine monoclonal antibody to significantly alter (+)-methamphetamine and (+)-amphetamine disposition in rats.

These studies examined the effects of a high-affinity anti-(+)-methamphetamine monoclonal antibody (mAb; KD = 11 nM) on (+)-methamphetamine [(+)-METH] and (+)-amphetamine [(+)-AMP] serum and tissue disposition and serum protein binding following i.v. (+)-METH administration. Male Sprague-Dawley rats were pretreated with a buffer solution (control rats) or with anti-(+)-METH mAb [equimolar in bi...